If you need this large volume for some reason, you might consider using an
autoclavable carboy with a magnetic stir bar for aeration and mixing. This
large volume will be difficult to handle though. I agree with the others,
your best bet would be a series of smaller flasks in a shaking incubator.
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Eric T. Gillock, Ph.D.
Associate Professor
Department of Biological Sciences
Fort Hays State University
Hays, KS 67601
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