[Microbiology] Re: need help-how to make bacterial mass inoculum

Larry Farrell via microbio%40net.bio.net (by farrlarr from isu.edu)
Tue Aug 5 11:28:04 EST 2008

Kanchanadevi k wrote:
> hi
> can you please tell me how to make bacterial culture in tub.
> actually, i will have to raise 5ltr of bacterial culture mass
> inoculum (Nutrient Broth)  in a tub for field application.
> so, if you have any idea about this
> please share with me
> Thanks a lot.
> K.Kanchana Devi
> Research Scholar

Why does it have to be in a tub?  If you are growing only 5 liters of 
culture, it would make much more sense to grow several smaller cultures 
(500 ml each in 1 liter flasks; 1 liter each in 2 liter flasks; etc.). 
It certainly isn't going to be easy to sterilize a tub, particularly 
given that most tubs available these days aren't made of materials that 
will withstand autoclave temperature.  Even if the tub is made of such 
material, it will be difficult to prepare a cover that will adequately 
protect the medium inside from contamination once it is removed from the 
autoclave (and I don't want to be around when you are trying to remove 
the tub/medium from the autoclave, or transport it to the field, without 
spilling it all over).  On the other hand, it is very easy to 
autoclave/move/inoculate 1 liter or 2 liter flasks (or even larger ones, 
if you have them).

Larry D. Farrell, Ph.D.
Professor Emeritus of Microbiology
Idaho State University
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