I had posted (several years back) a protocol for isolating plasmids up to
50kb. You may try and find that centrifugal protocol by using my name as a
key word. I used to use it routinly.
Quoting vikas agrawal <vikasagrawal56 from gmail.com>:
> Dear Roger,
> I am Vikas Agrawal, doing master's in India. I have been trying to isolate
> plasmid of 130 Kb size, but I am not getting the results. I have read your
> query at Bio.net. You were also facing the same problem. If you can tell me
> the procedure used to isolate large size plasmid DNA, I would be very
> thankful to you.
>> Thank You & Warm Regards.
> Vikas Agrawal.
> +91 9818894186
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Hiranya S. Roychowdhury, Ph.D.
Dept. of Health and Public Services, Rm# 191T,
Dona Ana Community College of New Mexico State University
Las Cruces, NM 88003