Larry,
I found a question you had answered regarding filamentous bacteria on
bio.net. I would really appreciate your help if you have a minute to
answer a question. I have isolated an organism from a polymer
emulsion (a raw material) that appears to be a filamentous gram neg
rod. This organism will only grow on low pH media and it grows very
slowly.
My question:
When I gram stain from a subculture after 24 hours growth the cells
are all free swimming with a few chains of cells. After 72 hours the
filaments are starting to form. The appearance of sheaths are
apparent at 72-96 hours. After 96 hours filaments are predominant,
most are sheathed.
Is it a proper technique when identifying filamentous bacteria to
observe older cultures? Am I correct in identifying this as a
filamentous bacteria?
Thank you so much!
Barbara
