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[Microbiology] Re: a question about vibrio cholerae

Sharad Sharma Sharad1982 at comcast.net
Sun Sep 24 22:59:57 EST 2006

hi judy
very good post to read - abt Vibrio
i started to pursue master's in microbiology this semester and i'll start
working in a QC microbiology lab soon (my first job in a biological/micro
field). it's amazing to read about and learn many different pathogens.
thanks for the links - i'd love to browse thru them.
You mentioned pathogenic microbiology course - i'm looking forward to that
one in the coming semesters.

take care



"JEDilworth" <bactitech at nospamhortonsbay.com> wrote in message 
news:qqidnUug1Iy6u5fYnZ2dnUVZ_v-dnZ2d at buckeye-express.com...
> Isolating Vibrios is basic medical microbiology. Like Tom Knight said, if 
> you're not familiar with the genus, you have some serious reading and 
> media making to do.
> What types of specimens are you using to isolate this organism? I have 
> worked in microbiology for nearly 30 years and have only heard of this bug 
> being isolated in our area (not an endemic area by any means) once in 
> thirty years. If you're in Indiana your climate is similar to mine, as I'm 
> in Ohio. You would need fecal specimens from outbreak or endemic areas.
> There are other Vibrios around also (V. parahemolytics and V. vulnificus).
> http://www.cdc.gov/search.do?action=search&queryText=Vibrio
> Vibrio usually occurs in areas where there is salt water. You need special 
> media, TCBS, to isolate it out of feces, assuming you even HAVE fecal 
> specimens that have it.
> http://www.emdchemicals.com/analytics/Micro_Manual/TEDISdata/prods/1_10263_0500.html
> All CAP accredited laboratories are required to have this media available 
> if the doctor is suspicious of a patient contracting Vibrios.
> In 2004, the CDC only had EIGHT isolates in the US.
> http://www.cdc.gov/foodborneoutbreaks/vibrio_sum/cstevibrio2004.pdf
> Half of the patients acquired this bug overseas. The others got it from 
> eating oysters in Georgia and Alabama. One third of the patients from 
> overseas died of their illness.
> I certainly hope you know what type of pathogen you're working with when 
> you mess with this bug. V. cholerae causes death by MASSIVE dehydration - 
> most of your bodily fluids just come out of your anus. They measure this 
> by putting patients on cholera cots (rubber cots with holes underneath to 
> catch the diarrhea in buckets so they can measure it) and try to infuse 
> you as fast as it's coming out. You essentially die of shock. I saw a film 
> of an Indian outbreak back in 1970 when I took a pathogenic microbiology 
> course in college. It's images were horrifying. People literally dried up. 
> They could grab a handful of skin from their abdomen and it would just 
> stand up and slowly sink back down, as there was no fluid in the cells.
> I've worked in clinical micro for nearly thirty years and wouldn't 
> particularly want to work with this bug. Messing with it casually in a 
> college laboratory is asking for trouble - sorry. Hopefully you have 
> biosafety cabinets to work under, and you wear gowns and gloves when 
> working with this.
> Judy Dilworth, M.T. (ASCP)
> Microbiology
> "h pardis2006" <h_pardis2006 at yahoo.com> wrote in message 
> news:mailman.1013.1158150749.20007.microbio at net.bio.net...
>> hi
>>  im looking for some help. we are working on isolation and identification 
>> and biochemical characterization of Vibrio cholera . we would approciate 
>> any comment . thanks alot in advance .

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