[Microbiology] transposon mutagenesis of B.pumilus

gholamreza ahmadian gholamrezaahmadian at yahoo.ca
Fri Sep 15 23:46:05 EST 2006

Dear Colleagues
I plan to use transposon mutagenesis to study the regulation of  chitinase operon in a natural isolate of Bacillus pumilus which is very similar to B.subtilis in biochemical characters. 
Does anybody have an experience in this field? Is there any  good kit which has worked before for you?
I appreciate 

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