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[Microbiology] Re: minimal medium for staphylococcus aureus (mrsa)

John Gentile via microbio%40net.bio.net (by yjgent from cox.net)
Thu Oct 26 18:44:39 EST 2006


Judy is right. Staph aureus will grow on just about any media, even 
simple trypticase soy agar - one of the simplest agars we use. However, 
if you need to isolate for it in a mixed bacterial load, then a 
selective agar is necessary. MSA has been mentioned, but if you are 
trying to target MRSA specifically then you need an Oxacillin containing 
media. We use ChromAgar MRSA from Becton Dikinson which isolates for 
MRSA and the suspect colonies grow with a unique color.

John Gentile, MS M(ASCP)

JEDilworth wrote:
> The question becomes: WHAT are you culturing? Are you setting up a culture 
> on something with mixed flora and need a medium to screen out other 
> organisms? Are you using this to store already isolated strains and you need 
> something cheap to keep stock cultures? Are you using the medium for 
> identification purposes or just for isolation? There are many different 
> choices out there. Are you looking to differentiate MRSA from other Staph. 
> aureus strains that are NOT MRSA?
> 
> Give us some more information, Prasanthi, and we may be able to help you out 
> a bit better.
> 
> Judy Dilworth, M.T.(ASCP)
> Microbiology
> 
>>prasanthi namburi wrote:
>>
>>>Dear microbiologists,
>>>    I am trying to find a recipie for the growth of staphylococcus aureus
>>>in a chemically defined minimal medium.
> 
> 
> 


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