"Bob" <bbx107 at excite.XXXX.com> wrote in message
news:bku542tqp733nq1ifsvgv38qftsouufaaj at 4ax.com...
> On Sat, 15 Apr 2006 22:32:49 -0700 (PDT), kondepudi kiran
> <kanthi_kiranin at yahoo.com> wrote:
>>>Dear Sir
>>>> My question is
>>>> 1. how to do electrphoresis and zymogram (activity satining of enzymes
>> on electrophoretic gels) of extracellular enzymes from obligate
>> halophiles since high NaCl possess problems in electrophoretic mobility.
>>>> 2. How to purify extracellular hydrolytic enzymes like amylase etc which
>> needs compulsory NaCl for the activity
>>>>> Why do you think there is a problem?? Unless the enzyme is
> irreversibly denatured by low salt, it would not be a problem. Do you
> know that it is?
>> Why is the salt a problem during purification??
You would think that lack of activity during purification would be a good
thing?
>> bob
>>
