Thanks for advice. I've tried Pfu:Taq in 1:10 proportion. In agarose it
looks great - comparing to my PCRs when I was using RedTaq (Sigma) I
have much more product (without cjangieng PCR program, 25 cyceles only)
I'm thinking if may be rhis result is even too good, cause more product
- more mistakes - even when I use high fidelity polymerase, am I right?
Soon I will sequence my products and we will see.