Well, I'll be the first to admit when I'm wrong. It looks like Pat is
right. Another thread (on another website) discusses this very same
issue.
http://www.protocol-online.org/archive/posts/12810.html
I have no idea how or why this protocol would work, but what do I know?
Someone on here was using a 10:1 ratio of Taq to Pfu.....so give it a
shot.
This protocol is very counter-intuitive (at least to me) on,
mechanistically, how these enzymes would cooperate to achieve a higher
fidelity reaction. Oh well.
--Alex
***************************
Alex B. Berezow, Grad Student
Dept. of Microbiology
University of Washington School of Medicine
Seattle, WA 98195
