[Microbiology] Re: Pfu polymerase mixed with Taq polymerase

biovirus04 at gmail.com biovirus04 at gmail.com
Sun May 28 02:42:26 EST 2006

Well, I'll be the first to admit when I'm wrong.  It looks like Pat is
right.  Another thread (on another website) discusses this very same


I have no idea how or why this protocol would work, but what do I know?
 Someone on here was using a 10:1 ratio of Taq to Pfu.....so give it a

This protocol is very counter-intuitive (at least to me) on,
mechanistically, how these enzymes would cooperate to achieve a higher
fidelity reaction.  Oh well.


Alex B. Berezow, Grad Student
Dept. of Microbiology
University of Washington School of Medicine
Seattle, WA  98195

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