That won't work. Pfu is a polymerase. It's not capable of
proofreading without polymerizing. So, it won't follow Taq around and
act as a high fidelity proofreader. If you mix the two enzymes, you'll
have some strands of DNA that are high fidelity (which were synthesized
by Pfu), and other strands that will be low fidelity (which were
synthesized by Taq). The only solution here, as I see it, is to only
use Pfu if you are needing high fidelity reactions.
--Alex
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Alex B. Berezow, Grad Student
Dept. of Microbiology
University of Washington School of Medicine
Seattle, WA 98195
