As Bob has already replied, a common problem. A technique long
used to give a crude approximation is Packed Cell Volume. Put a
representative sample of your culture into a centrifuge tube an spin
it to give a tight pellet, then estimate the volume of packed cells
(cubic centimeters) per unit volume. Unfortunately, if your culture
media contains a lot of solids, this creates a problem. And this does
not discriminate between live and dead cells. Tom McCloud
On Mon, 19 Jun 2006 18:46:17 +0300, gerchman at research.haifa.ac.il
wrote:
>Greetings all
>We are trying to measure the growth rate of rhizobium in different carbon
>sources but encountering dificulties. The main issue is that in liquide culture
>the bacteria seem to create "flakes" rather then homoginios turbidity, so
>absorbance seem out of the question. We thought about vortexing the sample and
>doing CFU count. Anyone tried this before? Any other ideas?
>Thanks Yoram
>>----------------------------------
>"Support bacteria, it's the only culture some people have"
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