hi, I have tried several times to transformation Bacillus, but most ended in vain, there appeared few or even no transformats when 10 ug plasmids were used.those who could point out the problems,as well as those could share his/her protocols with me, would be greatly appreciated.
Thanks in advance.
Centre for molecular virology,
Institute of Microbiology,
Chinese Academy of Sciences
Address: Room 414,East building,Beiyitiao No.13,Zhongguanchun,