Dear Professionals in microbiology,
I hope, you will be fine and healthy.
I am working on Boron Toxicity tolerance in Bacteria and I isolated several novel B-tolerant species. Now I am studying Boron Tolerance Mechanism, through B-uptake studies.
I want your kind help to understand an issue in my data. Kindly spare some moments from your busy schedule to see my data as here in the attached file "Data-uptake studies". Here I explain the problem that I could not understand although I repeated this experiment several times.
While doing B-uptake studies in bacteria (data is attached with figures, short introduction, and Materials and Methods and also with a short results and discussion), one species Bacillus boroniphilus (characterized and published just recently, please see characteristics of this species from the attached file "MS-boroniphilus") that requires B for its growth (unique character of this species in bacteriology) and at the same time, can tolerate very high concentration of B, showed more membrane (pallet)-bound B when exposed to low level of B supply than when exposed to high level (please see figure 1b, the last bars, page 7 in the attached data file). I could not understand the reasoning for this aspect. I repeated this experiment several times but always found same results i.e. more pallet-bound B when exposed to low B supply compared to when exposed to high B supply. In all other strains, the trend is opposite and understandable.
I would be thankful and obliged of your kindness and cooperation to extend my view for this data.
My best regards
Biotechnology Research Centre,
The University of Tokyo