The PCR route sounds like the best option so far.
Im interested to know how you know there are Ecoli present in your samples
if they are non cuturable.
<myrehfuss at ucdavis.edu> wrote in message
news:1155851145.838978.261920 at p79g2000cwp.googlegroups.com...
>biovirus04 at gmail.com wrote:
>> That's partially what I didn't get. Why would E. coli be
>> Stress and low pH can induce a VBNC state in E. coli. Perhaps NaCl and
> osmotic stress (or some other stress) in seawater can do likewise?
> VBNC E coli are apparently found in aquatic environments.
>> Another method of enumeration that requires no culture would be FISH
> using E coli specific probes.