[Microbiology] Re: methylene blue milk

Tony Stott GreenieLeBrun at hotmail.com
Mon Aug 14 18:27:04 EST 2006

JEDilworth wrote:
> I worked in a very small micro lab in the 70's and they were using a big
> pickle jar and a candle for their chocolate and Thayer Martin plates. It
> worked quite well. It was messy though, and somewhat tricky to position the
> candle and light it so you wouldn't burn yourself.
> I doubt whether it would be allowed in our more safety conscious
> laboratories now.
> I believe you meant 5% CO2 conditions, not anaerobic conditions. You need
> gas generators for the latter.
> Judy Dilworth, M.T. (ASCP)
> "N10" <limbic_lesion at hotmail.com> wrote in message
> news:NvidnbOphZo5rX3ZRVnyig at bt.com...
> > I remeber once just a few years back when we ran out of CO2 for the
> > genereation of  anaerobic conditions  I reverted to candling ! and it
> > worked very staisfactorily much to the amazement of the younger
> > microbiologists who witnessed it.

No, you need a brass anaerobic jar, a small metal mesh bag of palladium
catalyst, a water powered vacuum pump and basket ball bladder filled
from a cylinder of hydrogen, oh yes, don't forget to put your plates in
agar side down other wise the agar may fall into the lid when you apply
the vaccuum.

Anyone still know how to pull pasteur pipettes?

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