I am a lab researcher, I am working on enrichments of Iron reducers. WE are
using the FeOOH (hydroxide) as an electron acceptor: here is the deal: Fe in
our environment is present mostly as FeOOH, not as Fe3+.
I found the e-mail on internet and want to ask you a couple of questions.
I inferred from your message that you have an iron reducers culture. I have
a problem with these guys. While creating the media for them, I am using the
Fe2+ as an oxygen scavenger. THe thing is that the reduced agent I have been
using (thyglicolate) is so powerful, that it is reducig my FeOOH to green
rust (FeIII + FeII), thus actually doing the job of the iron reducers...
What can I do? I was trying to put only the Fe 2+, but it still does not
SO the question is: do you use pre-reduced media, (resazurine is
colorleess) or you use fust the regular pH 7 medium?
Department of Biology
University of Louisiana at Lafayette
Phone (Office): 482-5056