On Sat, 15 Apr 2006 22:32:49 -0700 (PDT), kondepudi kiran
<kanthi_kiranin at yahoo.com> wrote:
>Dear Sir
>> My question is
>> 1. how to do electrphoresis and zymogram (activity satining of enzymes on electrophoretic gels) of extracellular enzymes from obligate halophiles since high NaCl possess problems in electrophoretic mobility.
>> 2. How to purify extracellular hydrolytic enzymes like amylase etc which needs compulsory NaCl for the activity
>
Why do you think there is a problem?? Unless the enzyme is
irreversibly denatured by low salt, it would not be a problem. Do you
know that it is?
Why is the salt a problem during purification??
bob
