[Microbiology] Help needed

kondepudi kiran kanthi_kiranin at yahoo.com
Sun Apr 16 00:32:49 EST 2006

Dear Sir
  My question is
  1.  how to do electrphoresis and zymogram (activity satining of enzymes on electrophoretic gels) of extracellular enzymes from obligate halophiles since high NaCl possess problems in electrophoretic mobility.
  2. How to purify extracellular hydrolytic enzymes like amylase etc which needs compulsory NaCl for the activity
  Thanking you 
  Yours sincerely
  Kanthi Kiran.K

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