Historians believe that in newspost
<mailman.542.1144679535.16885.microbio at net.bio.net> on Mon, 10 Apr 2006,
Daniel Green <Daniel at york-bio.com> penned the following literary
masterpiece:
>I'm trying to find a suitable, high copy number plasmid for the
>expression of T7 RNA polymerase in E-Coli, (Minus the 6-His tag and
>associated probems that entails)
N-terminal His tagged T7 RNA pol expressed fine in our hands a few years
back and was simple to purify and worked fine. We used a modified pTTQ18
(http://tinyurl.com/rk2ml) with 6-His then an Nde I site.
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
