[Microbiology] Re: Request for help

Duncan Clark blackhole at abuse.plus.com
Mon Apr 10 10:39:27 EST 2006

Historians believe that in newspost 
<mailman.542.1144679535.16885.microbio at net.bio.net> on Mon, 10 Apr 2006, 
Daniel Green <Daniel at york-bio.com> penned the following literary 
>I'm trying to find a suitable, high copy number plasmid for the 
>expression of T7 RNA polymerase in E-Coli, (Minus the 6-His tag and 
>associated probems that entails)

N-terminal His tagged T7 RNA pol expressed fine in our hands a few years 
back and was simple to purify and worked fine. We used a modified pTTQ18 
(http://tinyurl.com/rk2ml) with 6-His then an Nde I site.

I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

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