On 9 Apr 2006 13:52:16 -0700, bhavyasai at gmail.com wrote:
>hi ,
>can anyone please tell me how to devise an enrichment medium for the
>culture of non fastidious facultatively acidophilic prokaryote able to
>fix atmospheric dinitrogen at 15c .where can we find such inoculum and
>what culture ingredients are reuired and what are the growth
>requirements.
>thanks,
Are you looking for an organism that is known, or for something new?
If the former, design the enrichment medium to favor its growth. If
the latter, you have to guess, or try more than one thing. The choice
of enrichment medium can bias the result, which is why it is
interesting. Do the enrichment differently, and you may come up with
something else.
Remember, enrichment media do not directly give you your desired
organism in pure culture. They merely increase its frequency -- enrich
for it. So you can design it based on what you know and what you guess
and what you would like -- and see what happens.
bob
