On 5 Apr 2006 15:36:15 -0700, bhavyasai at gmail.com wrote:
>hi,
>i have a qs:can arabinose and ampicillin be included in the
>differential or selective medium before it's autoclaved.if not describe
>a suitable method to sterilize these ingredients and also how and when
>they would be added to the medium.
>thanks
I don't know about arabinose specifically, but in general one can
autoclave a stock solution of a sugar -- but separately from the rest
of the medium. (Sugars are likely to react with other media
ingredients if autoclaved in the medium.) So, make (e.g.) a 20% (w/v)
solution of sugar, and autoclave it. Then add sugar solution to the
medium as needed.
Antibiotic solutions should be filter sterilized.
bob
