ahomosapien at gmail.com writes:
> Can anyone tell me whether fresh LB is better for bacterial growth than
> LB made the previous day, refrigerated overnight and used after warming
> to room temp? there should not be any problem, isnt it? IS any
> detrimental effect known at all?
LB composition and quality are poorly controlled. There is a great
deal of variation between batches of LB, which may explain differences
you have observed. We keep LB in the refrigerator for 2-3 months with
little apparent effect.