Hi everyone,
I am investigating the macromphiphiles diversity in High G+C Actinomycete
bacteria especially with the bacteria whose genome sequence have been fully
completed.
For this I need large mass of cells of that particular organism. I am having
some problem in growing Streptomyces species. As you know this organisms
grow well in fermentor, but our institution doesn't have any fermenter, so I
am trying to grow them in large conical flask in YEME media. But I can't use
any antifoam reagent as it may change the cell envelope structure which may
altered our macroamphiphiles. But without the antifoam it is very hard to
grow them in flask as I need 300 rpm for there growth.
So, please can anyone give any idea about growing Streptomyces in large
culture in limited time?
Cheers
Obaid.
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