Help an undergraduate student

The_Warrior almahdi at magma.ca
Fri Mar 4 23:34:37 EST 2005

My partner and I are so interested in some of the topics you have gave. The
first one that we choose is the one introduced by Mr. Robertson (the light
producing bacteria), the second one is the Aerobiology( the microbiology of
atmospheres) introduced by N10.
The third one we choose the organisms that are responsible for smelly feet.
Last but not least, Why not see if an array of common novel food
addatives  produce auxtrophic mutations  in ecoli. However, we thought of
using a fungus instead of an ecoli since it is more related to animals and
plants than bacteria in general.

As for Larry Farrell, We asked them for a bacteriaphage experiment, and the
instructor of the course said that we don't have good phages... I think they
want to my partner and I to do a literature based research, but I'm a very
practical person that like to work and do experiments.

Thanks again everyone, your help was like the cold pure water source from an
unexpected site in a harsh extremely hot desert...lol, looks too serious eh?
... but it was.

"N10" <limbic_lesion at hotmail.com> wrote in message
news:d08a89$739$1 at hercules.btinternet.com...
> Warrior
> Your a rounded person its so great to get a polite reply or even a reply
> Your gonna do fine  and if you get stuck you know where  to come :)
> Bets N10 :)
> "The_Warrior" <almahdi at magma.ca> wrote in message
> news:v7KdnUks1pa5OLrfRVn-3A at magma.ca...
> > Hello everyone, I truelly and honestly respect and honor your help, and
> > you
> > have provided me with very (I mean very) interesting ideas. I will
> > them with my instructor, and if he said "it is not challenging at all"
> > again, I'll beat him up !! haha.
> > Again, every one thank you soo much. I will tell you on which topic we
> > will
> > settle soon.
> >
> > Have a nice time and good luck with your studies.
> >
> > "Larry Farrell" <farrlarr at isu.edu> wrote in message
> > news:1109891845.bb34ea1f4a739d20fa8b565b01e5116a at teranews...
> >> The_Warrior wrote:
> >> > Hello every one, I'm a biology student in a University, and I truelly
> > need
> >> > your assistance in choosing a good topic for an undergraduate
> > microbiology
> >> > project.
> >> >
> >> Try isolating a bacterial virus (bacteriophage = phage) for your
> >> favorite bacterium.  Simply identify the bacterium to use and identify
> >> where it can be found in nature (E. coli and sewage samples work very
> >> well, if you make sure you get settled sewage that has not been
> >> chlorinated).  Collect a sample of that environmental material, suspend
> >> it is sterile water or use directly if it is an aqueous sample, mix
> >> an appropriate volume of 10X broth (of a medium that supports good
> >> growth of the bacterium to be used)and add a sample of a culture of
> >> bacterium.  Incubate overnight, centrifuge to remove bacteria, filter
> >> through a 0.45 micron filter and use the supernate as your phage stock.
> >>   Plate serial dilutions of the stock on your bacterium by the soft
> >> overlay method.  Pick isolated plaques and make new stocks of each and
> >> then compare plaque morphology, replication time for the phage, etc.
> >>
> >> If interested, I can supply additional specifics of the techniques, as
> >> can many other folks who lurk/post here.
> >> --
> >> Larry D. Farrell, Ph.D.
> >> Professor of Microbiology
> >> Idaho State University
> >
> >

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