Help an undergraduate student

N10 limbic_lesion at hotmail.com
Thu Mar 3 19:31:05 EST 2005


Your a rounded person its so great to get a polite reply or even a reply

Your gonna do fine  and if you get stuck you know where  to come :)

Bets N10 :)

"The_Warrior" <almahdi at magma.ca> wrote in message 
news:v7KdnUks1pa5OLrfRVn-3A at magma.ca...
> Hello everyone, I truelly and honestly respect and honor your help, and 
> you
> have provided me with very (I mean very) interesting ideas. I will discuss
> them with my instructor, and if he said "it is not challenging at all"
> again, I'll beat him up !! haha.
> Again, every one thank you soo much. I will tell you on which topic we 
> will
> settle soon.
> Have a nice time and good luck with your studies.
> "Larry Farrell" <farrlarr at isu.edu> wrote in message
> news:1109891845.bb34ea1f4a739d20fa8b565b01e5116a at teranews...
>> The_Warrior wrote:
>> > Hello every one, I'm a biology student in a University, and I truelly
> need
>> > your assistance in choosing a good topic for an undergraduate
> microbiology
>> > project.
>> >
>> Try isolating a bacterial virus (bacteriophage = phage) for your
>> favorite bacterium.  Simply identify the bacterium to use and identify
>> where it can be found in nature (E. coli and sewage samples work very
>> well, if you make sure you get settled sewage that has not been
>> chlorinated).  Collect a sample of that environmental material, suspend
>> it is sterile water or use directly if it is an aqueous sample, mix with
>> an appropriate volume of 10X broth (of a medium that supports good
>> growth of the bacterium to be used)and add a sample of a culture of your
>> bacterium.  Incubate overnight, centrifuge to remove bacteria, filter
>> through a 0.45 micron filter and use the supernate as your phage stock.
>>   Plate serial dilutions of the stock on your bacterium by the soft agar
>> overlay method.  Pick isolated plaques and make new stocks of each and
>> then compare plaque morphology, replication time for the phage, etc.
>> If interested, I can supply additional specifics of the techniques, as
>> can many other folks who lurk/post here.
>> --
>> Larry D. Farrell, Ph.D.
>> Professor of Microbiology
>> Idaho State University

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