I have a question regarding the Endospore staining procedure. We had an
exam in our Microbiology class, and we had to list all functions of
each of the steps in endospore staining. I did not put water as a
decolorizer, because it was never listed as such as all the other
decolorizers for staining as acetone alcohol is for grams stain, and
acid alcohol for acid-fast stains. I listed malachite green for
primary, heat as mordant, safranin as the countere stain. Any help or
links if I am indeed right water is not actually a decolorizer would be
appreciated.
Thanks in Advance.