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[Microbiology] Is water considered a decolorizer in Endospore staining?

bonaserat at yahoo.com bonaserat at yahoo.com
Sat Jul 16 00:06:43 EST 2005


I have a question regarding the Endospore staining procedure. We had an
exam in our Microbiology class, and we had to list all functions of
each of the steps in endospore staining. I did not put water as a
decolorizer, because it was never listed as such as all the other
decolorizers for staining as acetone alcohol is for grams stain, and
acid alcohol for acid-fast stains. I listed malachite green for
primary, heat as mordant, safranin as the countere stain. Any help or
links if I am indeed right water is not actually a decolorizer would be
appreciated. 

Thanks in Advance.



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