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[Microbiology] Re: Photographing bacteria in a Gram's stain

Scott Coutts scott.coutts at med.monash.edu.au
Sun Jul 10 05:34:02 EST 2005


First of Three of Six wrote:
> Hi can anyone share tips and tricks on capturing images of bacteria stained 
> by Gram's method.
> I'm using an Olympus BX51 with a DP70 and capturing the images using Olysia 
> BioReport.
> While I'm finding the process excellent for larger organisms via a 40 
> objective, when using oil 100 focusing and sharpness have suffered.
> Everything looks good on the screen, but then I transfer the image (saved as 
> tif and usually around 36 MB) 

What are you doing with them? You probably dont need to photograph them 
at such high resolution (well, with such large file sizes I should say).

 >
> and attempt some manipulation with Photoshop 
> to resize to 96 dpi and save as jpg (10/10 quality) the picture degrades a 
> little.
 >

Why do you want them at 96dpi? Do you need large image sizes? You may 
find that the images are better when recorded in lower resolutions to 
begin with if you dont need the print size.

 >
> Thanks in advance. I'm mostly keen to get any help on the best focussing 
> techniques people use.  
> 

Well, for focussing, I just focus until it's as sharp as I can get. Not 
much you can do once you've done that.

Scott.


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