Hi can anyone share tips and tricks on capturing images of bacteria stained
by Gram's method.
I'm using an Olympus BX51 with a DP70 and capturing the images using Olysia
While I'm finding the process excellent for larger organisms via a 40
objective, when using oil 100 focusing and sharpness have suffered.
Everything looks good on the screen, but then I transfer the image (saved as
tif and usually around 36 MB) and attempt some manipulation with Photoshop
to resize to 96 dpi and save as jpg (10/10 quality) the picture degrades a
Thanks in advance. I'm mostly keen to get any help on the best focussing
techniques people use.