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[Microbiology] Re: Spectro for growth curves

Scott Coutts scott.coutts at med.monash.edu.au
Mon Jul 4 06:39:42 EST 2005

Lesley Robertson wrote:
> "Scott Coutts" <scott.coutts at med.monash.edu.au> wrote in message 
> news:V4Mxe.12898$oJ.2999 at news-server.bigpond.net.au...
>>Hi Larry,
>>Are they the flasks with the sealed tube projecting from the neck? If so, 
>>yeah, I'd considered them, but it would be more convenient to have one 
>>that allows us to automate the procedure. Since we're buying new specs 
>>anyway, I thought it would be best to see if we can get a model that will 
>>do the widest range of things we need (: The thing is, I'm pretty sure 
>>they exist, I'm just not sure who makes them.
> There's a few things it would be useful to know - expected volumes, for a 
> start. How critical will dissolved oxygen be? Most of the automative reading 
> apparatus we had in the past  could only manage a very sedate shaking rate, 
> which meant that oxygen was frequently limiting as the cultures matured...
> Some years back, I was fortunate to use a spectrophotometer at the Free 
> University of Amsterdam which had been adapted to take a full-sized 
> continuous culture system (it was used for measuring cytochromes oxidation 
> levels at steady state) - 1 litre pot and all. I have an idea that they did 
> the adaptions themselves, but I'll try and check - that particular Prof has 
> now retired.


Thanks for your help. I've discovered a few systems, several from 
Thermo, several from Cecil, and one or two from Beckman. Most of them 
take samples from either a custom vessel that comes with the instrument 
(these are often actually designed for pharmaceutical tablet dissolution 
monitoring) or a user-supplied vessel. Others use a probe that can be 
inserted into any vessel. The best I've seen is a stirred, temperature 
controlled (peltier) cuvette that fits inside the spec.



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