[Microbiology] Filtration problem

Sly_76 sylvaincyr at hotmail.com
Tue Aug 16 15:41:33 EST 2005


I need to perform a sterility test by filtration (USP 71) on a solution
containing some particles (transport solution from fresh heart
recovered for heart valves transplant). So the particles seems to be
red blood cell, some proteins, lipids and all kind of stuff like that.
The initial solution is Ringer's Lactate solution (isotonic solution).
My problem is the .45um filter clogged. I tried a 20um prefilter but
the .45um clogged again. Then I tried 10um prefilter I was able to
filter 50ml (but i wish to filter 100ml). But if I use 10um or smaller
pore size filter, I will catch bugs (bacteria, fungi, mold) in the 10um
instead of the .45um (which one will be placed in broth for sterility
testing)... I will have to place in culture both filter... or what?...
What do you think about it?

Thank you...


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