I am doing a research work that deals with the detection of bacteria in
biological fluids like urine.
I will appreciate any help in finding a staining material that produces
fluorescence when reacting with bacteria. Fluorescence should be:
1) Intensive enough as to allow detection of bacteria concentration of at
least 10^3 cfu.
2) The excitation light should be sufficiently "far" and differentiated from
the emission light so that with proper light filtration, the excitaiton
light won't cause background noise to the emission light.
I am not talking about a microscopy method where you see stains but rather
about spectrophotometry that can detect fluorescent light and measure it.
Thanks in advance for your kind help.