HI TAnvir
As an alternative cant you just just "pure culture" the bugs from the
macophages and then extract their RNA..its a standard proceedure for
Ribotyping.
Best n10
""Md.Tanvir Rahman"" <rahmanm at uoguelph.ca> wrote in message
news:1095369724.414a03fcea206 at webmail.uoguelph.ca...
>I am trying to isolate RNA of bacteria from infected mammalian macrophage
> culture. When I isolated the RNA I can see some bands indicating
> presence of mammalian RNA inaddition to my bacterial RNA. I was wondering
> is
> there any way to isolate only the Bacterial RNA, not mammalian macrophage
> RNA
> from that infected culture!!
>> With thanks,
> Tanvir
> ---
