Im a student in Genetics from Austria. Im not using Newsgroups very
frequently but after spending hours on the Internet searching for an answer
to my question I finally choose this method to find what I´m looking for.
I hope some of you can hap me with this:
Why is it difficult to assay STAT/STAT Interaction with a classical Yeast -
Two - Hybrid System?
- Is it because of the SH2 Domains, that STATs have? (other
Transcriptionfactors do not have SH2 domains)
This implys that STATs have to be phosphyraleted for Dimerisation.
- Or is it because STATs must form Dimers to be translocated into the
nucleus. If this is the case I do not understand why, since I think it does
not matter whether Dimerisation happens before or after Translocation into
If anybody has the correct answer to my question or any suggestions please