Recombinant work with in AT rich bacillus DNA in E. coli

[Tom Knight]

You should know that lower extension temperatures are sometimes
required for PCR.  See

Su, X., Wu, Y., Sifri, C. D., & Wellems T. E., (1996).  Reduced
extension temperatures required for amplification of extremely
A+T-rich DNA.  Nucl Acid Res 24:1574-1575.