We have general problems with cloning and manipulations of Bacillus DNA
(AT-rich!) in E. coli (DH5 alpha), related to DNA instability, difficult
to clone, etc. We would be happy to know if anyone knows certain general
tricks (appropriate E. coli host, growth temperature, DNA preparation,
etc) that is useful when working with such DNA??
trygve
--
Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology
Email: trondaun at REMOVETHISBEFOREREPLYING!!biotech.ntnu.no
http://www.biotech.ntnu.no/molgen
- Must be sad being a dyslectic, agnostic insomniac, lying
awake during the night, wondering if there really is a dog
