HA tagging on E.coli chromosome

tracy dreamwestus at yahoo.com
Tue Jun 29 14:10:55 EST 2004


I am trying to chrosomally HA tag a gene on the chromosome in E.coli.  The
gene is the second gene in a three-gene operon, so I don't think it will be
good to insert a whole plasmid after the gene.  Does anybody have any
experience doing this?  What kind of suicide vector I can use in E.coli and
where can I get these vectors?  Or is it possilbe to tag this gene using
homologous recombination?  Any suggestion will be appreciated!  Thanks!

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