IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

HA tagging on E.coli chromosome

tracy dreamwestus at yahoo.com
Tue Jun 29 14:10:55 EST 2004


Hi,

I am trying to chrosomally HA tag a gene on the chromosome in E.coli.  The
gene is the second gene in a three-gene operon, so I don't think it will be
good to insert a whole plasmid after the gene.  Does anybody have any
experience doing this?  What kind of suicide vector I can use in E.coli and
where can I get these vectors?  Or is it possilbe to tag this gene using
homologous recombination?  Any suggestion will be appreciated!  Thanks!





More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net