I am trying to chrosomally HA tag a gene on the chromosome in E.coli. The
gene is the second gene in a three-gene operon, so I don't think it will be
good to insert a whole plasmid after the gene. Does anybody have any
experience doing this? What kind of suicide vector I can use in E.coli and
where can I get these vectors? Or is it possilbe to tag this gene using
homologous recombination? Any suggestion will be appreciated! Thanks!