Marcin Imielinski wrote:
>> Hello,
>> I have a pretty basic question for which I have had difficulty finding
> the answer to ...
>> What is the chemical composition of LB media? The product sheets
> generally list the "ingredients" as tryptone and yeast extract, and some
> list the composition in terms of amino acids and electrolytes. But it
> appears that given its messy origins this LB should be quite a bit more
> rich with metabolites. For example does LB contain nitrogenous bases or
> nucleotides? What carbohydrates are known to be present?
>
Well, you'd have to look for the chemical analysis of the individual
'ingredients'. As you say, in the lab, it's made up from trypton, yest
extract, salt and water. I assume you know what yeast extract and
trypton actually are? You're not going to get a complete and
comprehensive chemical analysis because these ingredients are too
complex. It's not a chemically defined medium!
Scott.
