IUBio

LB media chemical composition

Marcin Imielinski imielins at mail.med.upenn.edu
Thu Feb 12 18:06:31 EST 2004



Hello,

I have a pretty basic question for which I have had difficulty finding
the answer to ...

What is the chemical composition of LB media?  The product sheets
generally list the "ingredients" as tryptone and yeast extract, and some
list the composition in terms of amino acids and electrolytes.  But it
appears that given its messy origins this LB should be quite a bit more
rich with metabolites.  For example does LB contain nitrogenous bases or
nucleotides?  What carbohydrates are known to be present?

Thanks - 

Marcin Imielinski
University of Pennsylvania School of Medicine

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