"Vestitas" <vestitas at aol.com> wrote in message
news:20031015041824.15363.00000513 at mb-m23.aol.com...
> i've noticed that most bacterial ODs are measured at 600nm this being a
> wavelength at which the light is scattered and not absorbed by the cells
(?)
> therefore the higher the OD the more turbid the culture. So why do some
people
> measure OD at 660nm? - Please help, I'm confused
We always did it at 430. It doesn't really matter what you use as long as
you standardize everything, use a good blank and make sure that you're
measuring over a range where your OD meter is linear.
Lesley Robertson
http://www.beijerinck.bt.tudelft.nl
