IUBio

bacterial OD

Vestitas vestitas at aol.com
Wed Oct 15 03:18:24 EST 2003


i've noticed that most bacterial ODs are measured at 600nm this being a
wavelength at which the light is scattered and not absorbed by the cells (?)
therefore the higher the OD the more turbid the culture.  So why do some people
measure OD at 660nm? - Please help, I'm confused



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