IUBio

Flourescent stain for Ralstonia?

EK nobody at elnino.com
Thu Oct 2 11:57:23 EST 2003


Group of Alexander Steinbuchel has been working on developing methods of
staining bacteria, producing polyhydroxyalkanoates and other inclusion
bodies. I remember, Nile Blue, Nile Red, as well as tetrazolium violet
worked quite well. Check out e.g.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9914303&dopt=Abstract

Emir

"Adam Lawrence" <adamlaw at yahooFNORD.com> wrote in message
news:3f7c2cf6$0$578$b45e6eb0 at senator-bedfellow.mit.edu...
> Hi-
>
> Does anyone know of a blue or green fluorescent stain that will
> selectively stain the cell wall or cell membrane of gram-negative
> bacteria (Ralstonia eutropha)?  Or does anyone have any guesses on
> stains that might work or companies I might try?  I struck out with
> Molecular Probes and google is driving me nuts!
>
> Thanks!
> Adam
>





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