Well, I think a better group would be one of the molecular biology
groups, in particular bionet.molbio.methds-reagnts.
But anyhow, I dont have a protocl, but have you checked with the
manufacturer of the chondroitinase? They probably have something. Call
their tech support people. Who are you buying it from?
Cameron Beck wrote:
> Howdy all. I'm new to the whole newsgroup thing, but I thought I'd give it
> a try. I'm a graduate student at BYU, working with Dr. Robert Seegmiller on
> his continuing osteoarthritis studies. Anyway, I'm trying to put together a
> protocol for immunostaining of degraded proteoglycans in the articular
> cartilcage of mouse knee joints. All the literature I've read refers to a
> preliminary incubation with chondroitinase ABC to remove chondroitin sulfate
> and allow the antibody better access to the degradation neoepitopes. I
> haven't been able to locate a protocol for this yet though. Does anyone
> have a suggestion as to where I could find one? Many thanks.