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Chondroitinase ABC

Scott Coutts scott.coutts at med.monash.edu.au
Thu May 22 06:48:43 EST 2003

Well, I think a better group would be one of the molecular biology 
groups, in particular bionet.molbio.methds-reagnts.

But anyhow, I dont have a protocl, but have you checked with the 
manufacturer of the chondroitinase? They probably have something. Call 
their tech support people. Who are you buying it from?

Cameron Beck wrote:
> Howdy all.  I'm new to the whole newsgroup thing, but I thought I'd give it
> a try.  I'm a graduate student at BYU, working with Dr. Robert Seegmiller on
> his continuing osteoarthritis studies.  Anyway, I'm trying to put together a
> protocol for immunostaining of degraded proteoglycans in the articular
> cartilcage of mouse knee joints.  All the literature I've read refers to a
> preliminary incubation with chondroitinase ABC to remove chondroitin sulfate
> and allow the antibody better access to the degradation neoepitopes.  I
> haven't been able to locate a protocol for this yet though.  Does anyone
> have a suggestion as to where I could find one?  Many thanks.

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