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Chondroitinase ABC

Cameron Beck nedbucket at hotmail.com
Tue May 20 18:14:26 EST 2003


Howdy all.  I'm new to the whole newsgroup thing, but I thought I'd give it
a try.  I'm a graduate student at BYU, working with Dr. Robert Seegmiller on
his continuing osteoarthritis studies.  Anyway, I'm trying to put together a
protocol for immunostaining of degraded proteoglycans in the articular
cartilcage of mouse knee joints.  All the literature I've read refers to a
preliminary incubation with chondroitinase ABC to remove chondroitin sulfate
and allow the antibody better access to the degradation neoepitopes.  I
haven't been able to locate a protocol for this yet though.  Does anyone
have a suggestion as to where I could find one?  Many thanks.





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