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Cannot get ride of gDNA before ReverseT PCR

Duncan Clark junk at hgmp.mrc.ac.uk
Sat May 17 05:44:37 EST 2003


Historians believe that in newspost <3ECF0946 at sws.ncl.ac.uk> on Thu, 15 
May 2003, HMRomero-Arce@?.?.invalid penned the following literary 
masterpiece:
>My problem is that now
>that I want to digest the DNA to get only the RNA for RT-16SPCR

Why???

There are no introns in 16S bacterial RNA so you will get the same 
sequence from PCRing genomic DNA as one would using RT-PCR. No need to 
go to RNA and RT-PCR unless you have a very specific reason for doing 
so.

I have severe doubts that you will have intact RNA from a soil prep 
anyway so you may actually get nothing with RT-PCR.

If you really really want to go down the RNA route then try Ambion's new 
Turbo DNase I

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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