Hello, hope someone can give me some advice.
I did DNA/RNA soil extraction following Griffitts, 2000 method of
extraction.
Then I stored the extracted DNA/RNA in 1x TE buffer. My problem is that now
that I want to digest the DNA to get only the RNA for RT-16SPCR it's
impossible to digest completely the DNA. I read that there is a limited
amount
of DNA that can be digested in the DNase assay. To start with, my samples
got
a lot of DNA, they were stored in a buffer containing EDTA and I cannot
modify
the protocol for extraction because by now population in the soil has
changed
so I need to work with these extracts. Dilution is not an option because
that
would cause the lost of RNA which belongs to minority species in the soil.
I'd like to know if a part from the many kits that are in the market to
purify
RNA from gDNA there is a 'lab-made' way of doing so, taking in account the
state of my extracts.
Thank you.
Monica
