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fermentation of coli and plasmid stability

S.Ballal ballal_sv at hotmail.com
Tue May 13 20:59:43 EST 2003


Ede,

thats the way we also test the stability.  But maybe it was just our
luck that  we did not face any problems.

There are other ways to test,that is by colony blotting and transfer 
followed by probing with suitable antibodies against your protein  Or 
using a DNA probe for your gene.

You can also pick few colonies ( may be 25 or even 100 ) and carry out
a PCR with probes against your gene of interest.

None of these are as simple as plating methods but these for sure will
tell you if you have a actual loss of plasmid or they are not just
coming up on the plates ( let me think why such a thing would
happen!!!)

Regards,

S.Ballal
Indus Biotherapeutics Ltd.
Ahmedabad, INDIA
http://www.indusbio.co.in


Ede <bggfg at gmx.de> wrote in message news:<9po2cv84558u5h8o5rslguudcqaateeevi at 4ax.com>...
> we checked the stability by plating out diluted cultures on selective
> and non selective media. How you check your stability.
> Ede
> >



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