You can check out del Solar et al., 1998: "Replication and control of
circular bacterial plasmids", Bingle and Thomas (2001): "Regulatory
circuits for plasmid survival". They may be a bit too theoretical but
they may contain valuable references.
Trond Erik
Trond Erik Vee Aune wrote:
>>> Ede wrote:
>>> Hi there,
>> is anybody there who can give me an idea, how i can solve the problem
>> of low plasmid stability during a fermentation in coli. The problem is
>> i cannot select with antibiotics. If somebody knows how i can increase
>> the stability of my plasmid it would be great. also if somebody have a
>> good review or so about this problem
>>> You could try using some kind of partition (par) or killer system (i.e.
> hok/sok). Partition systems (mostly found on low-copy plasmids, I think)
> make sure that at least a copy of each plasmid is partitioned to each
> cell at division. Killer systems kill off cells without plasmid. In this
> way it's better to use a partitioning system than a killer system, since
> the effect of the last one reduces growth rate.
>> I'll look for references when I get to school.
>> Trond Erik
>
--
Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology
Email: trondaun at biotech.ntnu.nohttp://www.REMOVETHISBEFOREREPLYING!!biotech.ntnu.no/molgen
