Well, you dont even have to look in places others havent looked... I
think I mentioned this before, but anyhow - we found one growing as a
contaminant on a new agar plate in our cold room!
Trond Erik Vee Aune wrote:
>>> JEDilworth wrote:
>>> Hate to say I told you so........but it just didn't sound like a gram
>> negative to
>> me.
>>> Nah, don't feel bad about it. I'm just glad I learnt how to do 16s
> sequencing. Now I'm going to look for new bacteria, I just need to find
> some place where other people haven't been looking before.
>> Btw, I'm still working with the Ganges bacteria (Paenibacillus alvei).
> It seem to have a plasmid of less than 30kb, so I'm going to see if I
> can establish it in some other bacteria and see if some of the phenotype
> from the Paenibacillus is transferred to the new one, for instance
> antibiotics production. I need some practical work to do inbetween
> reading to exam :)
>> Trond Erik
>>>>>> Bacillus will only sporulate under adverse conditions. If you're
>> providing it with
>> breakfast, lunch, and dinner on a great plate, it has no need to form
>> spores.
>>>> Judy Dilworth, M.T. (ASCP)
>> Microbiology (Clinical) 28 years
>>>> Trond Erik Vee Aune wrote:
>>>>>>> Yes, it was highly motile and swarmed in a very fascinating way. That's
>>> why I decided to identify it. I looked for spores in the microscope, but
>>> couldn't see any, so maybe the cells were in the wrong growth phase.
>>>>>> Trond Erik
>>>>>>>>>>>> Anyway, I got very good match against Paenibacillus alvei.
>>>>>> According to
>>>>>>>>>>>>>>> web-resources this genus do produce different types of antibiotics as
>>>>> well as being able to swarm in different ways
>>>>> (http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange
>>>>> thing
>>>>> is that it is not a gram negative bug as I thought after staining, but
>>>>> one of you microbiologists suggested that I was a Bacillus strain,
>>>>> so I
>>>>> guess we geneticists are much better at sequencing than staining ;)
>>>>> Anyway, I'm pretty pleased that I finally got an identification,
>>>>> but of
>>>>> course a little disappointed that it wasn't a new bacterium
>>>>>>>>>>>
