Ede wrote:
> Hi there,
> is anybody there who can give me an idea, how i can solve the problem
> of low plasmid stability during a fermentation in coli. The problem is
> i cannot select with antibiotics. If somebody knows how i can increase
> the stability of my plasmid it would be great. also if somebody have a
> good review or so about this problem
You could try using some kind of partition (par) or killer system (i.e.
hok/sok). Partition systems (mostly found on low-copy plasmids, I think)
make sure that at least a copy of each plasmid is partitioned to each
cell at division. Killer systems kill off cells without plasmid. In this
way it's better to use a partitioning system than a killer system, since
the effect of the last one reduces growth rate.
I'll look for references when I get to school.
Trond Erik
